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KMID : 1007520210300050711
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Food Science and Biotechnology
2021 Volume.30 No. 5 p.711 ~ p.721
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Antiproliferative and antibacterial activity of extracts of Ganoderma strains grown in vitro
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Serrano-Marquez Leonardo
Trigos Angel
Couttolenc Alan
Padron Jose M.
Shnyreva Alla V.
Mendoza Guillermo
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Abstract
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In this bioprospecting study the biological activities of extracts of the in vitro culture of Ganoderma Mexican strains were evaluated. The extracts were tested by the Sulforhodamine B staining method for antiproliferative activity and the plate microdilution method for antibacterial activity. Extracts that proved bioactive in these two activities, the antioxidant activity (Galvinoxyl, ABTS, and DPPH) and total phenolic contents (Folin-Ciocalteu) were additionally determined, as well as acute toxicity (Artemia franciscana). In the antiproliferative activity Ganoderma curtisii strain (GH-16-015) obtained a remarkable value of GI50?≤?50 μg/mL against tumor lines: A549, HBL-100, HeLa, and T-47D. G. curtisii strains (GH-16-012 and GH-16-015) showed MIC values?=?500 μg/mL against Staphylococcus aureus. G. curtisii strain (GH-16-012) almost reduced by 50% the radical Galvinoxyl. Finally, G. curtisii strain (GH-16-023) presented the lowest level of toxicity with a LC50 of 490.881 μg/mL against A. franciscana. These results support the potential medicinal effects of Mexican strains of G. curtisii.
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KEYWORD
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Antibacterial activity, Antioxidant activity, Antiproliferative activity, Brine shrimp, Molecular identification
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